Rogozyan B.N., Efimenko N.V., Mikhailova N.Yu.

JSC «Anapa» health centre", Anapa, Balneological Research Institute of Ministry of Health, Pyatigorsk, Russia

The study highlights the effects of serial applications of green and brown algae on animals in comparison to curative mud.

Objects of the study: green and brown algae preparations, curative mud, Wistar rats.

We used immunologic and biochemical essays.

Statistically significant data of there immunologic and biochemical essays are obtained. Significant differences among essays are found in many parameters depending on types of algae.

Results: effects of green and brown algae in intact animals’ initiates’ formation of new physiological chain systems aimed at optimizing of regulatory processes.

We studied immune effects of serial applications of green and brown algae preparations in comparison to classic Anapa curative silt mud in experiments on animals. Wistar rats were the objects of this study. We investigated immunologic essays (leukogram, white blood cell count, level of circulating immune complexes (CIC) in blood, indices of phagocytosis by neutrophils (phagocytic activity of leukocytes (PAL, leukocytic index of phagocytosis (LIP), index of complete phagocytosis (ICP), tetrazolium blue tests — spontaneous (TBTsp) and induced (TBTin), blast-cell transformation reactions of lymphocytes — spontaneous (BCTRLsp), induced by phithemagglutinin (BCTRL-PGA) and by concanavalin A (BCTRL-ConA).

Biochemical essays: levels of whole protein, fibrinogen, insulin, cortisol, serotonin, histamine, epinephrine, dopamine in blood. All animals were divided into 4 groups: 1st — 14 intact rats; 2nd — 12 rats who received mud applications on thymus region (12 procedures within 1 month with 1-3 days interval, temperature of applications was 42ºC); 3rd — 13 rats who received applications of milled green algae on thymus region (the same regimen as in the 2nd group); 4th group — 13 rats who received applications of milled brown algae on thymus region (the same regimen as in the 2nd group). WBC count significantly altered after applications of Anapa curative silt mud. However, special attention should be given to abrupt raise of blood lymphocyte count in the group which received applications of brown algae (p<0.001). This increase is much more marked than in Anapa mud group. In full accordance to these phenomenon levels of polysegmentonuclear neutrophils in blood decreased. TBT essays: after applications of green algae its stimulation degree significantly increased against ‘spontaneous’ level. Weights of spleen and thymus as well as count of antibody producing cells (APC) in spleen and blood level of CIC in animals from investigated groups were approximately equal. However we should note the phenomenon of abrupt amplification of BCTRL-PGA in animals after applications of brown algae; this phenomenon disappears when mitogen is substituted by ConA. Drastic increase of cellularity of spleen and thymus after applications of both types of algae is of the highest interest. This phenomenon is absent after applications of Anapa curative silt mud.

In comparison to Anapa mud, brown algae significantly activated production of serotonin and dopamine, and inhibited secretion of histamine. Whole protein level in blood significantly increased after applications, especially of green algae. All types of applications produced almost no effect on blood levels of insulin. Catabolic effect of hypercortisolemia which is characteristic for Anapa mud applications was not observed after applications of algae.

Special attention should be given to new-formed relations associated with algae applications:

- green algae applications: between APC and thymus weight and dopamine; BCTRL and dopamine and insulin levels; spleen weight and blood levels of whole protein and epinephrine; cellularity of thymus and whole protein level in blood; whole protein and cortisol; insulin and serotonin.
- brown algae applications: between CIC and dopamine and cortisol; BCTRL and epinephrine and whole protein; thymus weight and cellularity of thymus and spleen; cellularity of thymus and epinephrine; cortisol and dopamine.
These results evidence that applications of green and brown algae in intact animals initiate formation of new physiological chain systems, most of which is aimed at optimizing of regulatory processes in immune, hormonal and other biochemical constellations.

Summarizing the results of studies of effects of green and brown algae applications we can conclude that each of these effects has its specificity in general trend of activation of immune and biochemical processes. This property can be used for primary and secondary prophylaxis of widespread diseases.